6AZY

Crystal structure of Hsp104 R328M/R757M mutant from Calcarisporiella thermophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 65
Unit cell lengths	139.173, 139.173, 97.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.337 - 2.700
R-factor	0.2005
Rwork	0.198
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qvr
RMSD bond length	0.008
RMSD bond angle	1.167
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2276))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.096	0.000
Number of reflections	29450	1467
<I/σ(I)>	23.6	2.25
Completeness [%]	100.0	100
Redundancy	7.3	7.1
CC(1/2)		0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.2 M Ca acetate, 0.1 M MES/NaOH, pH 6.0, 10% propanol, 5mM ADP, 10 mM MgCl2, cryo 35% glycerol