6AZM
Crystal structure of the 580 germline antibody bound to circumsporozoite protein NANP 5-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.961, 166.555, 72.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.511 - 1.600 |
| R-factor | 0.1668 |
| Rwork | 0.166 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 580 Fab Crystal structure |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.862 |
| Data reduction software | XDS |
| Data scaling software | SHELXPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rpim | 0.046 | 0.397 |
| Number of reflections | 113396 | 18628 |
| <I/σ(I)> | 13.8 | 2.2 |
| Completeness [%] | 98.8 | 98.6 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 10% Isopropanol, 100mM HEPES pH 7.5, 20% PEG 4000 |
