6AX3

Complex structure of JMJD5 and Symmetric Dimethyl-Arginine (SDMA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.456, 64.706, 77.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.637 - 2.250
R-factor	0.2114
Rwork	0.206
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fbj
RMSD bond length	0.009
RMSD bond angle	1.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.640
High resolution limit [Å]	2.250
Number of reflections	11947
<I/σ(I)>	14.6
Completeness [%]	97.0
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M HEPES pH 7.0 8% PEG 3350