6AU4
Crystal structure of the major quadruplex formed in the human c-MYC promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.663, 69.811, 32.952 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.906 - 2.350 |
R-factor | 0.2229 |
Rwork | 0.220 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wo2 1xav |
RMSD bond length | 0.009 |
RMSD bond angle | 1.080 |
Data reduction software | XDS (Nov 1, 2016) |
Data scaling software | XDS (Nov 1, 2016) |
Phasing software | PHASER ((1.10.1_2155: ???)) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.030 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rpim | 0.013 | 0.167 |
Number of reflections | 6774 | |
<I/σ(I)> | 34 | 4.4 |
Completeness [%] | 99.4 | 99.7 |
Redundancy | 12.6 | 12.9 |
CC(1/2) | 1.000 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 20% MPD 300mM KCl 50mM LiCl 50mM Sodium cacodylate |