6ATK
Crystal structure of the human coronavirus 229E spike protein receptor binding domain in complex with human aminopeptidase N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-02-24 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 153.820, 153.820, 322.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.795 - 3.505 |
R-factor | 0.2452 |
Rwork | 0.244 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fyq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.701 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Rpim | 0.080 | 0.330 |
Number of reflections | 55989 | 5490 |
<I/σ(I)> | 10.9 | 2.7 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 4.1 | |
CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8% PEG 8000, 1mM GSSG, 1mM GSH, 5% Glycerol, 100mM MES, 1ug/mL endo-beta-N-acetylglucosaminidase A |