6ASZ

Chromodomain HP1 with Y24F mutation bound to histone H3 peptide containing trimethyl lysine

Replaces:  5KOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	34.516, 76.780, 75.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.894 - 1.518
R-factor	0.2561
Rwork	0.255
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kne
RMSD bond length	0.006
RMSD bond angle	0.749
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	10.894
High resolution limit [Å]	1.518
Number of reflections	15582
<I/σ(I)>	4.4
Completeness [%]	98.2
Redundancy	5.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.3	277	0.1 M MES, 3.4 M (NH4)2SO4