6ASV
E. coli PRPP Synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.757, 138.436, 137.438 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.210 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dah |
RMSD bond length | 0.005 |
RMSD bond angle | 1.013 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
High resolution limit [Å] | 2.210 | 6.020 | 2.210 |
Rmerge | 0.067 | 0.034 | 0.526 |
Rmeas | 0.075 | 0.038 | 0.595 |
Rpim | 0.033 | 0.017 | 0.272 |
Number of reflections | 49727 | 2649 | 2349 |
<I/σ(I)> | 6.8 | ||
Completeness [%] | 99.3 | 99.1 | 95.1 |
Redundancy | 4.7 | 4.7 | 4.4 |
CC(1/2) | 0.999 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Magnesium Chloride 0.1 M Hepes, pH 7.5 30% w/v PEG 400 1 mM ADP |