6ARV
Crystal structure of CARM1 with Compound 2 and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.230, 99.120, 208.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.780 - 2.000 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.348 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.780 | 70.778 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.031 | 0.761 | |
| Rmeas | 0.097 | 0.036 | 0.871 |
| Rpim | 0.045 | 0.017 | 0.417 |
| Number of reflections | 105501 | 1285 | 7736 |
| <I/σ(I)> | 11.9 | 14.8 | 0.9 |
| Completeness [%] | 99.5 | 96.7 | 99.9 |
| Redundancy | 4.7 | 4.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG3350 |
