6ARQ
Crystal structure of CD96 (D1) bound to CD155/necl-5 (D1-3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.138, 70.819, 224.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.550 - 2.880 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fqp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.870 | 2.990 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.145 | 0.619 |
| Rpim | 0.092 | 0.387 |
| Number of reflections | 11730 | 1620 |
| <I/σ(I)> | 6.4 | 1.8 |
| Completeness [%] | 87.7 | 85.3 |
| Redundancy | 3.1 | 3.2 |
| CC(1/2) | 0.984 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 32% (v/v) Jeffamine ED-2001 0.1M HEPES pH7.3 |
