6AQR
SAGA DUB module Ubp8(C146A)/Sgf11/Sus1/Sgf73 bound to monoubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.755, 103.177, 112.846 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.150 - 2.100 |
R-factor | 0.2036 |
Rwork | 0.201 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mhs |
RMSD bond length | 0.020 |
RMSD bond angle | 1.855 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER (2.6.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.920 | 46.920 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.093 | 0.052 | 0.572 |
Rmeas | 0.103 | 0.057 | 0.630 |
Rpim | 0.043 | 0.024 | 0.259 |
Total number of observations | 304961 | 3983 | 24852 |
Number of reflections | 53790 | 761 | 4370 |
<I/σ(I)> | 13.3 | 35.9 | 3 |
Completeness [%] | 99.2 | 94.8 | 99.7 |
Redundancy | 5.7 | 5.2 | 5.7 |
CC(1/2) | 0.996 | 0.995 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES pH 7.0 17% PEG3350 0.1 M ammonium sulfate |