6APN
HLA-A*0201 single chain trimer with HPV.16 E7 peptide LLMGTLGIV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2014-09-18 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.363, 65.094, 130.321 |
| Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.220 |
| R-factor | 0.21582 |
| Rwork | 0.213 |
| R-free | 0.26005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mrk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.131 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.010 |
| High resolution limit [Å] | 2.220 |
| Number of reflections | 37686 |
| <I/σ(I)> | 12.5 |
| Completeness [%] | 90.7 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M MES, pH 6,5, 25% PEG 2000 MME |
