6APL
Crystal Structure of human ST6GALNAC2 in complex with CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.18076 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.168, 71.125, 138.608 |
| Unit cell angles | 103.69, 97.26, 103.03 |
Refinement procedure
| Resolution | 41.605 - 2.350 |
| R-factor | 0.2001 |
| Rwork | 0.196 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | the structure of apo enzyme the associated PDB entry |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.729 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.059 | 0.595 |
| Rpim | 0.042 | 0.421 |
| Number of reflections | 120969 | 6277 |
| <I/σ(I)> | 19.8 | 1.2 |
| Completeness [%] | 97.5 | 96 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 277 | 0.1M Sodium Citrate (pH 4.2), 0.1M Ammonium Sulfate, and 24% (w/v) PEG 20000 |
