6APL
Crystal Structure of human ST6GALNAC2 in complex with CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-14 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.18076 |
Spacegroup name | P 1 |
Unit cell lengths | 71.168, 71.125, 138.608 |
Unit cell angles | 103.69, 97.26, 103.03 |
Refinement procedure
Resolution | 41.605 - 2.350 |
R-factor | 0.2001 |
Rwork | 0.196 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | the structure of apo enzyme the associated PDB entry |
RMSD bond length | 0.004 |
RMSD bond angle | 0.729 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.059 | 0.595 |
Rpim | 0.042 | 0.421 |
Number of reflections | 120969 | 6277 |
<I/σ(I)> | 19.8 | 1.2 |
Completeness [%] | 97.5 | 96 |
Redundancy | 1.7 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.2 | 277 | 0.1M Sodium Citrate (pH 4.2), 0.1M Ammonium Sulfate, and 24% (w/v) PEG 20000 |