6AOT
Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin L194P mutant in complex with 6'-SLNLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.337, 100.337, 384.341 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.18355 |
| Rwork | 0.183 |
| R-free | 0.20294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.552 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.030 | 0.280 |
| Number of reflections | 55042 | 4982 |
| <I/σ(I)> | 44.6 | 3.4 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 9.1 | 8.7 |
| CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.1 M CAPS pH 10.5 and 29% PEG 400 |
