6AOK
Crystal structure of Legionella pneumophila effector Ceg4 with N-terminal TEV protease cleavage sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.035, 48.027, 100.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.172 - 1.880 |
| R-factor | 0.1585 |
| Rwork | 0.156 |
| R-free | 0.19840 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2733) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.071 | 0.525 |
| Rpim | 0.035 | 0.266 |
| Number of reflections | 16374 | 817 |
| <I/σ(I)> | 21.4 | 3.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.2 M magnesium chloride, 0.5 mM manganese chloride, 0.1 M Tris pH 7.3, 30% (w/v) PEG 4K, 2 mM phosphotyrosine |
