6AOB

Crystal structure of non-canonical dimeric guanylyl cyclase domain of RhoGC fusion protein from the aquatic fungus Blastocladiella emersonii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	91.769, 95.880, 100.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.966 - 1.700
R-factor	0.1657
Rwork	0.164
R-free	0.19630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ao9
RMSD bond length	0.006
RMSD bond angle	0.759
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.106	1.300
Number of reflections	48364
<I/σ(I)>	16.1	2
Completeness [%]	99.3	97.7
Redundancy	12.8	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 200 mM potassium sodium tartrate