6ANK
Synaptotagmin-7, C2A- and C2B-domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.637, 75.059, 70.980 |
Unit cell angles | 90.00, 114.51, 90.00 |
Refinement procedure
Resolution | 44.456 - 2.254 |
R-factor | 0.218 |
Rwork | 0.214 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n5a 6anj |
RMSD bond length | 0.014 |
RMSD bond angle | 0.489 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.460 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.079 | 0.766 |
Rpim | 0.046 | |
Number of reflections | 25080 | |
<I/σ(I)> | 15.4 | 1.29 |
Completeness [%] | 91.6 | 56.3 |
Redundancy | 3.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, 0.1 M calcium chloride, 0.125 M KCl, 20% ethylene glycol |