6AL4

CRYSTAL STRUCTURE OF ANTI-CD19 ANTIBODY B43 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	73.100, 127.360, 219.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.20645
Rwork	0.206
R-free	0.23642
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i15
RMSD bond length	0.005
RMSD bond angle	1.016
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.470
High resolution limit [Å]	2.380	2.380
Rmerge	0.071	0.563
Number of reflections	80381	7505
<I/σ(I)>	22.3	3.4
Completeness [%]	96.9	91.7
Redundancy	5.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.1	293	20% PEG 3350, 0.2 M NA PHOSPHATE DIBASIC