6AKP

Crystal Structural of FOXC2 DNA binding domain bound to PC promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	85
Detector technology	CCD
Collection date	2016-12-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	41.837, 80.214, 101.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.283 - 2.323
R-factor	0.2204
Rwork	0.217
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x07
RMSD bond length	0.004
RMSD bond angle	0.650
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.290	2.407
High resolution limit [Å]	2.323	2.323
Rmerge	0.129	0.917
Number of reflections	15111	1366
<I/σ(I)>	13.97
Completeness [%]	98.0
Redundancy	13.4
CC(1/2)	0.994	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM NaOAc pH4.7, 14%PEG4K, 200mM NaCl, 10mM MgCl2, 0.5mM TCEP