6AJ5
Crystal structure of ligand-free type DHODH from Eimeria tenella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 248.808, 248.808, 248.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.27586 |
| Rwork | 0.272 |
| R-free | 0.35881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w7r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.796 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.132 | 0.850 |
| Number of reflections | 32346 | 1587 |
| <I/σ(I)> | 5.6 | 2.62 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, 3.0 M ammonium sulfate |
