6AGO

Crystal structure of MRG15-ASH1L Histone methyltransferase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 31 1 2
Unit cell lengths	115.757, 115.757, 209.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.917 - 3.103
R-factor	0.2235
Rwork	0.220
R-free	0.27230
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.064	0.033	0.736
Rmeas	0.071	0.037	0.830
Rpim	0.030	0.016	0.373
Total number of observations	147885
Number of reflections	29386	1554	1461
<I/σ(I)>	8
Completeness [%]	99.9	99.6	99.9
Redundancy	5	4.8	4.6
CC(1/2)		0.998	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		295	Ammonium Acetate