6AGL
Molecular basis for feedback inhibition of tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 87.240, 48.722, 89.897 |
Unit cell angles | 90.00, 117.91, 90.00 |
Refinement procedure
Resolution | 37.921 - 2.500 |
R-factor | 0.244 |
Rwork | 0.242 |
R-free | 0.28290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kfl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.589 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.049 | 0.358 |
Number of reflections | 22959 | 1885 |
<I/σ(I)> | 16 | |
Completeness [%] | 97.8 | |
Redundancy | 5.1 | |
CC(1/2) | 0.996 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 24% PEG3350 and 0.2 M KF, number 23 of PEG/Ion |