6AGK

The structure of CH-II-77-tubulin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97973
Spacegroup name	P 21 21 21
Unit cell lengths	105.084, 157.062, 182.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.21398
Rwork	0.211
R-free	0.26399
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ezy
RMSD bond length	0.008
RMSD bond angle	1.352
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.084	2.400
Number of reflections	75447
<I/σ(I)>	20
Completeness [%]	99.9
Redundancy	6.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1M MES, 30MM CACL2, 30MM MGCL2, PH 6.7,