6AG5
Crystal structure of Ard1 N-terminal acetyltransferase E88H/H127E mutant from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-12-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9765 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.217, 52.926, 73.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.314 - 2.320 |
R-factor | 0.1691 |
Rwork | 0.160 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.194 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.320 |
High resolution limit [Å] | 2.320 | 2.300 |
Number of reflections | 7313 | |
<I/σ(I)> | 10.69 | |
Completeness [%] | 99.4 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 20% Tacsimate pH 7.0 |