6AE3
Crystal structure of GSK3beta complexed with Morin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2015-06-27 |
| Detector | RIGAKU |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.588, 134.360, 100.393 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.140 |
| R-factor | 0.19236 |
| Rwork | 0.191 |
| R-free | 0.22626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.485 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 93509 | |
| <I/σ(I)> | 38.17 | |
| Completeness [%] | 97.8 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 100mM Sodium Citrate (pH6.5), 18%(v/v) PEG 4000, 5%(v/v) 2-propanol |
