6ADC
Crystal structure of the E148A mutant CLC-ec1 in the presence of 50mM bromoacetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.249, 96.732, 169.779 |
| Unit cell angles | 90.00, 131.45, 90.00 |
Refinement procedure
| Resolution | 34.852 - 3.055 |
| R-factor | 0.2281 |
| Rwork | 0.226 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ene |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.302 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 3.160 |
| High resolution limit [Å] | 3.055 | 3.050 |
| Rmerge | 0.150 | 1.330 |
| Number of reflections | 102941 | 10254 |
| <I/σ(I)> | 32.1 | 1.6 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | PEG400 24% (w/v), 100mM glycine, 20 mM Na/K tartrate, 50 mM bromoacetate |
