6ACO
histone lysine desuccinylase Sirt5 in complex with succinyl peptide H2BK120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.885, 64.877, 55.564 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 55.270 - 1.710 |
| R-factor | 0.16353 |
| Rwork | 0.161 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ace |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.270 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.089 | 0.355 |
| Number of reflections | 23736 | 4727 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 92.3 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate trihydrate (pH 6.5), 30%(w/v) PEG8000 |
