6ACE
histone lysine desuccinylase Sirt5 in complex with succinyl peptide H3K122
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.227, 41.169, 55.854 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 61.410 - 1.980 |
| R-factor | 0.20609 |
| Rwork | 0.203 |
| R-free | 0.26634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3riy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.850 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.410 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.107 | 0.516 |
| Number of reflections | 18297 | 674 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 92.6 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1M sodium cacodylate (pH 5.5), 25%(w/v) PEG 4000 |
