6AC0

Crystal structure of TRADD death domain GlcNAcylated by EPEC effector NleB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	CCD
Collection date	2013-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97899
Spacegroup name	P 41 21 2
Unit cell lengths	28.600, 28.600, 264.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.435 - 1.449
R-factor	0.1864
Rwork	0.184
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oq9
RMSD bond length	0.006
RMSD bond angle	0.873
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	1.530
High resolution limit [Å]	1.449	1.449
Rmerge	0.111	0.349
Number of reflections	20061	2913
<I/σ(I)>	8.1	3.8
Completeness [%]	96.7	99.3
Redundancy	4.6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1.6 M Na/KPO4, pH 6.0