6AAW
Mdm2 in complex with a D amino Acid Containing Stapled Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.946, 43.378, 34.542 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 40.330 - 2.000 |
| R-factor | 0.18637 |
| Rwork | 0.184 |
| R-free | 0.22336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4umn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.202 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PARROT |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.330 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.246 | 1.211 |
| Rmeas | 0.267 | 1.324 |
| Rpim | 0.105 | 0.528 |
| Number of reflections | 8132 | 1118 |
| <I/σ(I)> | 5.7 | 3.4 |
| Completeness [%] | 99.3 | 95.2 |
| Redundancy | 6.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20 % w/v Polyethylene glycol 8,000, 100 mM HEPES pH 7.5, 200 mM Ammonium sulfate, 10 % v/v 2-Propanol |
