6AAD
Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with mTmdZLys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-28 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.917, 43.782, 72.475 |
Unit cell angles | 90.00, 119.05, 90.00 |
Refinement procedure
Resolution | 35.019 - 1.444 |
R-factor | 0.1716 |
Rwork | 0.171 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zim |
RMSD bond length | 0.012 |
RMSD bond angle | 1.374 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmeas | 0.074 | |
Rpim | 0.027 | 0.283 |
Number of reflections | 48373 | 2317 |
<I/σ(I)> | 34.2 | |
Completeness [%] | 97.4 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | Tris-HCl buffer (pH 8.5),PEG 200, KCl, MgCl2, sodium citrate |