6A8C
Ribokinase from Leishmania donovani with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 61 |
| Unit cell lengths | 100.700, 100.700, 126.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.140 - 1.980 |
| R-factor | 0.17164 |
| Rwork | 0.170 |
| R-free | 0.20924 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a8a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.140 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.139 | 0.537 |
| Number of reflections | 50454 | 27089 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 296 | 0.1M Citric acid, 3.4M Sodium chloride, 10mM Magnesium chloride, 9% Glycerol |
