6A8B
Ribokinase from Leishmania donovani with AMPPCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-01-28 |
Detector | RIGAKU AFC-5R |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 100.395, 100.395, 125.785 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.790 - 2.010 |
R-factor | 0.18604 |
Rwork | 0.184 |
R-free | 0.22592 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a8a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.676 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.790 | 2.060 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.113 | 0.661 |
Number of reflections | 47985 | 3514 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 99.9 | |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 296 | 0.1M Citric acid pH 4.3, 3.4M Sodium chloride, 10mM Magnesium chloride, 10% Glycerol |