6A7C
Human dihydrofolate reductase complexed with NADPH and BT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.942, 82.942, 77.489 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.350 - 2.060 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ddr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.493 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 12165 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 2.7 M Ammonium sulphate, 100 mM K2HPO4, 3%(v/v) Ethanol |
