6A73
Complex structure of CSN2 with IP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.804, 70.164, 81.350 |
| Unit cell angles | 107.03, 103.94, 90.25 |
Refinement procedure
| Resolution | 20.003 - 2.447 |
| R-factor | 0.2503 |
| Rwork | 0.247 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1L17 and 4ZWJ |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.003 | 2.490 |
| High resolution limit [Å] | 2.447 | 2.450 |
| Rmerge | 0.025 | 0.189 |
| Number of reflections | 27516 | 1305 |
| <I/σ(I)> | 26.1 | |
| Completeness [%] | 91.8 | 87.6 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulfate ,100mM Bis-Tris pH 5.5, 25%PEG 3350. |
