6A6X
The crystal structure of the Mtb MazE-MazF-mt9 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.975, 70.975, 156.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.604 - 2.700 |
R-factor | 0.2137 |
Rwork | 0.211 |
R-free | 0.27060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wyg |
RMSD bond length | 0.005 |
RMSD bond angle | 0.794 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.209 | 0.922 |
Number of reflections | 11645 | 1125 |
<I/σ(I)> | 20.9 | 4.85 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 12.5 | 12 |
CC(1/2) | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M MES (pH5.5) and 1.3-1.5 M ammonium sulfate |