6A6I
Crystal structure of the winged-helix domain of Cockayne syndrome group B protein in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.161, 101.705, 66.008 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 39.960 - 2.600 |
| R-factor | 0.1814 |
| Rwork | 0.178 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.094 | |
| Number of reflections | 21604 | 1229 |
| <I/σ(I)> | 7.4 | 1.2 |
| Completeness [%] | 99.6 | 95.3 |
| Redundancy | 5.4 | 2.6 |
| CC(1/2) | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 28% PEG1000, 10% glycerol, 0.1 M tricine (pH 8.0), and 350 mM MgCl2. |
