6A6E

Crystal structure of thermostable Cysteine desulfurase (FiSufS) from thermophilic Fervidobacterium Islandicum AW-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 2
Unit cell lengths	106.302, 218.716, 89.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.755 - 2.090
R-factor	0.14387
Rwork	0.142
R-free	0.18685
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3i
RMSD bond length	0.018
RMSD bond angle	1.853
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.755	2.140
High resolution limit [Å]	2.095	2.100
Rmerge	0.097	0.524
Number of reflections	122276
<I/σ(I)>	26.84
Completeness [%]	98.7
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M diAmmonium hydrogen citrate, 20% PEG 3350, pH 5.1