6A4W
AcrR from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2014-05-03 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 61 |
Unit cell lengths | 118.154, 118.154, 90.906 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.536 - 2.587 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a4l |
RMSD bond length | 0.006 |
RMSD bond angle | 1.331 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.587 | 2.600 |
Rpim | 0.053 | 0.282 |
Number of reflections | 21205 | 1086 |
<I/σ(I)> | 28.7 | |
Completeness [%] | 94.3 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1260 mM Ammonium sulfate 100 mM MES/ Sodium hydroxide pH 6.0 |