6A4L

AcrR from Mycobacterium tuberculosis

Summary for 6A4L

• Entry DOI: 10.2210/pdb6a4l/pdb
• Descriptor: TetR family transcriptional regulator, SULFATE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: transcription regulator, transcription
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 2
• Total formula weight: 52401.95

Authors

Kang, S.M. (deposition date: 2018-06-20, release date: 2019-06-26, Last modification date: 2024-10-16)

Primary citation

Kang, S.M.,Kim, D.H.,Jin, C.,Ahn, H.C.,Lee, B.J.
The crystal structure of AcrR from Mycobacterium tuberculosis reveals a one-component transcriptional regulation mechanism.
Febs Open Bio, 9:1713-1725, 2019

PubMed Abstract: Transcriptional regulator proteins are closely involved in essential survival strategies in bacteria. AcrR is a one-component allosteric repressor of the genes associated with lipid transport and antibiotic resistance. When fatty acid ligands bind to the C-terminal ligand-binding cavity of AcrR, a conformational change in the N-terminal operator-binding region of AcrR is triggered, which releases the repressed DNA and initiates transcription. This paper focuses on the structural transition mechanism of AcrR of Mycobacterium tuberculosis upon DNA and ligand binding. AcrR loses its structural integrity upon ligand-mediated structural alteration and bends toward the promoter DNA in a more compact form, initiating a rotational motion. Our functional characterization of AcrR and description of the ligand- and DNA-recognition mechanism may facilitate the discovery of new therapies for tuberculosis.

PubMed: 31369208
DOI: 10.1002/2211-5463.12710

Experimental method

X-RAY DIFFRACTION (2.8 Å)