6A4U
The first crystal structure of crustacean ferritin that is a hybrid type of H and L ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-18 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 0.8 |
| Spacegroup name | I 4 |
| Unit cell lengths | 124.872, 124.872, 175.683 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.590 - 1.160 |
| R-factor | 0.1387 |
| Rwork | 0.138 |
| R-free | 0.15660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ajo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.056 | 0.464 |
| Rpim | 0.036 | 0.327 |
| Number of reflections | 456412 | 22818 |
| <I/σ(I)> | 26.9 | |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35% MPD, 100mM imidazole-HCl pH 8.0 and 200mM MgCl2 |
