6A3A

MVM NES mutant Nm2 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	106.650, 106.650, 304.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.990 - 2.300
R-factor	0.2111
Rwork	0.209
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.005
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.119	0.065
Rmeas	0.125	0.068
Rpim	0.045	0.020	0.867
Total number of observations	972120
Number of reflections	78675	4344	3843
<I/σ(I)>	2.8
Completeness [%]	99.6	99.9	99
Redundancy	12.4	11.7	12.7
CC(1/2)		0.997	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6