6A2J
Crystal structure of heme A synthase from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 15 |
Detector technology | PIXEL |
Collection date | 2017-12-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 90.465, 90.465, 147.309 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.031 - 2.200 |
R-factor | 0.2076 |
Rwork | 0.206 |
R-free | 0.22870 |
Structure solution method | MIRAS |
RMSD bond length | 0.017 |
RMSD bond angle | 2.010 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE (2.13) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.031 | 29.031 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.126 | 0.048 | 1.230 |
Rmeas | 0.140 | 0.053 | 1.372 |
Number of reflections | 22834 | 720 | 3385 |
<I/σ(I)> | 8.02 | 26.88 | 1.2 |
Completeness [%] | 99.9 | 99.6 | 99.7 |
Redundancy | 5.242 | 5.244 | 5.092 |
CC(1/2) | 0.997 | 0.997 | 0.494 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 30% PEG 600, 0.1M sodium chloride, 0.1M lithium sulfate, 0.1M sodium HEPES pH 7.4 |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 30% PEG 600, 0.1M sodium chloride, 0.1M lithium sulfate, 0.1M sodium HEPES pH 7.4 |