6A10
Crystal structure of hen egg white lysozyme crystallized by ammonium sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 41.399, 57.971, 57.618 |
Unit cell angles | 115.97, 105.20, 98.81 |
Refinement procedure
Resolution | 19.295 - 1.130 |
R-factor | 0.164 |
Rwork | 0.163 |
R-free | 0.17610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wld |
RMSD bond length | 0.011 |
RMSD bond angle | 1.365 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.150 |
High resolution limit [Å] | 1.130 | 3.070 | 1.130 |
Rmerge | 0.042 | 0.022 | 0.359 |
Rmeas | 0.060 | 0.032 | 0.508 |
Rpim | 0.042 | 0.022 | 0.359 |
Total number of observations | 293247 | ||
Number of reflections | 151134 | 5732 | 7388 |
<I/σ(I)> | 12.8 | ||
Completeness [%] | 91.4 | 69.4 | 89.1 |
Redundancy | 1.9 | 1.9 | 1.9 |
CC(1/2) | 0.995 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.2M ammonium sulfate, 6%(v/v) 1,4-dioxane |