6A0I
Crystal structure of human protein N-terminal asparagine amidohydrolase (NTAN1) C75S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.982, 84.340, 86.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.527 - 1.996 |
R-factor | 0.1662 |
Rwork | 0.164 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a0e |
RMSD bond length | 0.005 |
RMSD bond angle | 0.726 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rpim | 0.048 | 0.247 |
Number of reflections | 42029 | 2075 |
<I/σ(I)> | 16.31 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | PEG 3350, potassium phosphate monobasic |