6ZXS
Cold grown Pea Photosystem I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-08-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 190.197, 201.786, 213.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.630 - 3.000 |
| R-factor | 0.24 |
| Rwork | 0.239 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l8r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.754 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.326 | |
| Number of reflections | 162077 | 7998 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 7 | |
| CC(1/2) | 0.997 | 0.066 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | di-potassium phosphate, Tris, PEG400, glycerol, L-glutathione, octyl glucose neopentyl glycol |
