6ZSI
The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.919532 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.360, 35.380, 165.670 |
Unit cell angles | 90.00, 93.76, 90.00 |
Refinement procedure
Resolution | 46.108 - 1.914 |
R-factor | 0.2252 |
Rwork | 0.223 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5szi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.231 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.108 | 46.108 | 1.960 |
High resolution limit [Å] | 1.910 | 8.560 | 1.910 |
Rmerge | 0.068 | 0.032 | 0.872 |
Rmeas | 0.071 | 0.034 | 0.948 |
Total number of observations | 591373 | ||
Number of reflections | 52601 | 659 | 3671 |
<I/σ(I)> | 19.75 | 61.69 | 2.07 |
Completeness [%] | 99.5 | 99.1 | 93.3 |
Redundancy | 11.243 | 12.382 | 6.47 |
CC(1/2) | 0.999 | 0.999 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M Mes pH 6.0 5% w/v PEG 3000 30% v/v PEG 200 |