6ZQO

EYFP mutant - F165G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	57.888, 57.888, 168.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 2.200
R-factor	0.2585
Rwork	0.254
R-free	0.33960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfp
RMSD bond length	0.006
RMSD bond angle	1.542
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.060	0.040	0.794
Rmeas	0.066	0.044	0.876
Rpim	0.028	0.019	0.362
Total number of observations	83201
Number of reflections	14573	1475	1457
<I/σ(I)>	9.8
Completeness [%]	94.7	85.6	98.4
Redundancy	5.7	5.1	5.5
CC(1/2)		0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5.7 mg/ml in 20mM Tris 8.0 200 mM NaCl mixed with a 1.44M (NH4)2SO4, 60mM Bicine pH 9.0 reservoir solution.