6ZP8
Yeast 20S proteasome in complex with glidobactin-like natural product HB335
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.160, 300.120, 144.830 |
| Unit cell angles | 90.00, 113.11, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cz4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.095 | 0.520 |
| Number of reflections | 210818 | 19807 |
| <I/σ(I)> | 10.87 | 2.4 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
