6ZNV

Protein polybromo-1 (PB1 BD2) Bound To DP28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	C 1 2 1
Unit cell lengths	83.706, 50.370, 33.582
Unit cell angles	90.00, 97.43, 90.00

Refinement procedure

Resolution	41.500 - 1.140
R-factor	0.1928
Rwork	0.192
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hmf
RMSD bond length	0.005
RMSD bond angle	0.826
Data reduction software	xia2
Data scaling software	Aimless (7.0.078)
Phasing software	PHASER (7.0.078)
Refinement software	REFMAC (7.0.078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.500	1.180
High resolution limit [Å]	1.140	1.140
Number of reflections	43994	2536
<I/σ(I)>	24.7	2.7
Completeness [%]	87.2
Redundancy	6.9
CC(1/2)	0.998	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	0.005 M zinc acetate 20% (w/v) PEG 3350