6ZMD
Crystal structure of HYPE covalently tethered to BiP bound to AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976250 |
Spacegroup name | P 41 |
Unit cell lengths | 83.466, 83.466, 169.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.470 | 2.770 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.057 | 0.567 |
Number of reflections | 34024 | 4518 |
<I/σ(I)> | 10.1 | 1.9 |
Completeness [%] | 100.0 | |
Redundancy | 3.7 | |
CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M NaCl, 0.1 M Na/K phosphate pH 6.2 and 10% (w/v) PEG 8000 |